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resource source identifier antibodies anti gfap primary antibody proteintech
Resource Source Identifier Antibodies Anti Gfap Primary Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and morehttps://www.bioz.com/product/resource+source+identifier+antibodies+anti+gfap+primary+antibody+proteintech/MAP2+Antibody/pm36936073-205-2-9
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1) Product Images from "A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution."
Article Title: A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.
Journal: Cell reports methods
doi: 10.1016/j.crmeth.2023.100407
Figure Legend Snippet: Figure 4. Compatibility of VALID with 2D and 3D immunolabeling (A) Fluorescence images of different antibodies co- labeled with VALID, including parvalbumin (PV), glial fibrillary acidic protein (GFAP), tyrosine hydroxylase (TH), NeuN, ionized calcium binding adapter molecule 1 (Iba1), and microtubule-asso- ciated protein 2 (MAP2). (B and C) 3D reconstruction of the labeled NeuN- positive cellsandVALID-castedvascular structures, respectively. Merged images shown in (B) and (C). (D) Optical sections of reconstructed images shown in (C) at different imaging depths.
Techniques Used: Immunolabeling, Fluorescence, Labeling, Binding Assay, Imaging
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Recombinant:Article Title: A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFAP primary antibody Proteintech Cat16825-1-AP, RRID:AB_2109646 Goat anti-rabbit secondary antibody Alexa Fluor 488 Abcam Cat#ab150077, RRID:AB_2630356 Anti-CD31 Alexa Fluor 647 BioLegend Cat#102416, RRID:AB_493410 Anti-Tyrosine Hydroxylase Rabbit pAb Servicebio Cat#GB11181, RRID: AB_2921651 NeuN Polyclonal antibody Proteintech Cat#26975-1-AP, RRID:AB_2880708 Parvalbumin Polyclonal antibody Proteintech Cat#29312-1-AP, RRID: AB_2918280 CD31 Mouse Monoclonal antibody Proteintech Cat#66065-2-Ig, RRID: AB_2918476 MAP2 antibody Proteintech Cat# 17490-1-AP, RRID:AB_2137880 Iba1 antibody Abcam Cat# ab5076, RRID:AB_2224402 Chemicals, peptides, and recombinant proteins PBS Sigma-Aldrich Cat#P3813 Paraformaldehyde Sigma-Aldrich Cat#158127 Pork skin gelatin Sigma-Aldrich Cat#V900863 DiI Aladdin Cat#D131225 DiO Aladdin Cat#D131213 DiD Thermo Fisher Scientific Cat#D7757 MeOH Sinopharm Chemical Reagent Co. Ltd. Cat#10014118 EtOH Sinopharm Chemical Reagent Co. Ltd. Cat#10009218 Tert-butanol Sigma-Aldrich Cat#360538 Tetrahydrofuran Sigma-Aldrich Cat#186562 DBE Sigma-Aldrich Cat#108014 MXDA Tokyo Chemical Industry Cat#D0127 Sorbitol Sigma-Aldrich Cat#85529 Triton X-100 Sigma-Aldrich Cat#T8787 L. esculentum (Tomato) lectin Vector Laboratories Cat#DL1178 Sodium azide Sigma-Aldrich Cat#S2002 DAPI Thermo Fisher Scientific Cat# 62248 Wheat germ agglutinin, Alexa Fluor TM 647 conjugate Thermo Fisher Scientific Cat# W32466 Evans Blue Sigma-Aldrich Cat# E2129 Experimental models: Organisms/strains C57BL/6J Jackson Laboratories Cat# JAX:000664, RRID:IMSR_JAX:000664 Software and algorithms Matlab Mathworks https://www.mathworks.com/ products/matlab.html Imaris Bitplane https://www.bitplane.com/ imaris/imaris Fiji 55 https://imagej.nih.gov/ij/ Other Conical tubes (50 mL) Corning Cat#430828 Conical tubes (15 mL) Corning Cat#430790 (Continued on next page) Cell Reports Methods 3, 100407, February 27, 2023 e1 ..
Article Title: A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFAP primary antibody Proteintech Cat16825-1-AP, RRID:AB_2109646 Goat anti-rabbit secondary antibody Alexa Fluor 488 Abcam Cat#ab150077, RRID:AB_2630356 Anti-CD31 Alexa Fluor 647 BioLegend Cat#102416, RRID:AB_493410 Anti-Tyrosine Hydroxylase Rabbit pAb Servicebio Cat#GB11181, RRID: AB_2921651 NeuN Polyclonal antibody Proteintech Cat#26975-1-AP, RRID:AB_2880708 Parvalbumin Polyclonal antibody Proteintech Cat#29312-1-AP, RRID: AB_2918280 CD31 Mouse Monoclonal antibody Proteintech Cat#66065-2-Ig, RRID: AB_2918476 MAP2 antibody Proteintech Cat# 17490-1-AP, RRID:AB_2137880 Iba1 antibody Abcam Cat# ab5076, RRID:AB_2224402 Chemicals, peptides, and recombinant proteins PBS Sigma-Aldrich Cat#P3813 Paraformaldehyde Sigma-Aldrich Cat#158127 Pork skin gelatin Sigma-Aldrich Cat#V900863 DiI Aladdin Cat#D131225 DiO Aladdin Cat#D131213 DiD Thermo Fisher Scientific Cat#D7757 MeOH Sinopharm Chemical Reagent Co. Ltd. Cat#10014118 EtOH Sinopharm Chemical Reagent Co. Ltd. Cat#10009218 Tert-butanol Sigma-Aldrich Cat#360538 Tetrahydrofuran Sigma-Aldrich Cat#186562 DBE Sigma-Aldrich Cat#108014 MXDA Tokyo Chemical Industry Cat#D0127 Sorbitol Sigma-Aldrich Cat#85529 Triton X-100 Sigma-Aldrich Cat#T8787 L. esculentum (Tomato) lectin Vector Laboratories Cat#DL1178 Sodium azide Sigma-Aldrich Cat#S2002 DAPI Thermo Fisher Scientific Cat# 62248 Wheat germ agglutinin, Alexa Fluor TM 647 conjugate Thermo Fisher Scientific Cat# W32466 Evans Blue Sigma-Aldrich Cat# E2129 Experimental models: Organisms/strains C57BL/6J Jackson Laboratories Cat# JAX:000664, RRID:IMSR_JAX:000664 Software and algorithms Matlab Mathworks https://www.mathworks.com/ products/matlab.html Imaris Bitplane https://www.bitplane.com/ imaris/imaris Fiji 55 https://imagej.nih.gov/ij/ Other Conical tubes (50 mL) Corning Cat#430828 Conical tubes (15 mL) Corning Cat#430790 (Continued on next page) Cell Reports Methods 3, 100407, February 27, 2023 e1 ..
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